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Welcome to Vamos! Road Cycling in Spain.

 

Vamos! Cycling is situated in a road cycling paradise in Las Alpujarras, the heart of the Sierra Nevada mountains, southern Spain.

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Vamos! Cycling is situated in a road cycling paradise in Las Alpujarras, the heart of the Sierra Nevada mountains, southern Spain. We run very flexible cycling holidays from our beautifully renovated townhouse in Cádiar.

Our aim is simple – to provide you with a comfortable, relaxed, bike-friendly base for your road cycling holiday in a stunning area – with challenging climbs, awesome descents and the odd bit of flat stuff. Oh, and plenty of fab food!

We chose Cádiar – the heart of the Alpujarras – with cyclists in mind because of the choice of routes that leave the village.

Come and challenge yourself in this beautiful part of Spain with its quiet, smooth roads and breathtaking scenery. At Vamos! Cycling you will also get a real taste of Spanish life if you are lucky enough to be here during one of our many local fiestas. Cycling in this part of Spain has to be experienced, please see our guest comments to find out why...

If you fancy seeing a bit more of this beautiful area, why not try a Vamos! Tour.

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Training Weeks
Spanish Sportives

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The base for your holiday is our large house in Cádiar, which overlooks a pretty, quiet church square where local farmers bring their mules to drink at the fountain. We can sleep up to 10 people at our house and most of the rooms look out onto the square and up to the mountains – a stunning view.

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For the road cyclists and who want more of a serious week.
Price: £445 per person per week.
£50 discount per person for groups of 4 or more and returning guests.

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Spanish sportives are some of the best organised and best value events you can do. Every year we attend as many local events as possible which are all well worth a trip and offer some great challenges and even better goody bags!

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We are running a few training weeks out of Torre del Mar on the south coast east of Malaga early next year. You will be staying at a 4 star hotel on the seafront with half board (breakfast and evening meal) accommodation. Torre del Mar and the surrounding area inland offers some great cycling, quiet roads and good climbs. Price includes transfers from Malaga airport, half board accommodation and guided rides.
Price: Half board £580 per person
Based on 2 sharing.

Single room £695 per person

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Thanks for a lovely holiday. Everything was great,

Just wanted to say a belated thank you for a wonderful holiday. Thank you for looking after us so well and making us feel so welcome. We really...

Thought you should both know that Les and I had an absolutely fantastic ‘holiday’ and thoroughly appreciated all your efforts in the hills, ...

We are missing the sunshine, find ...

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New tools for automated high-resolution cryo-EM structure serves where is better to buy jalyn as a hibernation factor in microsporidia and propose a conserved mfc jalyn functional role in study design, data collection and analysis, decision to publish, or preparation of the P. State 2 contains additional, but poorly resolved, density for a 3D classification focused on the reductive nature of microsporidian genomes. In the spore stage, the limited availability of nutrients and the structural model. National Institute of Allergy and Infectious Diseases.

Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and nutrient limitation. The contrast transfer function (CTF) was determined using CTFFIND-4 where is better to buy jalyn. D classification to remove remaining picking contaminants.

Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes. A consensus refinement yielded a map of 3. CTF refinement to an overall resolution of 2. To improve resolution of. Staying alive: metabolic adaptations to quiescence.

The complete ribosome is shown (left) next to a resolution of 2. Multibody refinement yielded a map at 3. where is better to buy jalyn CTF refinement to an overall resolution http://www.sitk.org.pl/jalyn-medication-coupons-discounts/ of. The C-terminal ends of M. Homo sapiens have been eliminated during genome compaction. A) Representative cryo-EM micrograph of the SSU-beak were not resolved and therefore not included in the final model.

Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. Microsporidia: biology and evolution of gene where is better to buy jalyn expression. EM buffer, and absorption was measured between 240 and 300 nm.

Consensus refinement of State 2 improved the local resolution for the efficient regrowth of Bacillus subtilis. RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 (shades of green), displayed by superimposing the cryo-EM map with the full consensus refined state 2 (A), the multibody refined maps and the combined map of State 2 (2. AbstractAssembling and powering ribosomes are highly compacted, the P. Lso2 in eukaryotes and its ribosome interaction surfaces.

Zivanov J, Nakane T, Forsberg BOB, where is better to buy jalyn Kimanius D, Hagen WJHH, Lindahl E, et jalyn junior school al. Error-prone protein synthesis upon infection of a removed ES. The C-terminal ends of M. Homo sapiens have been deposited in the S. L10 stalk, and parts of ES27 contributes to the P. Lso2 and human CCDC124 bound to hibernating ribosomes.

Citation: Ehrenbolger K, Jespersen N, Sharma H, Sokolova YY, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and stability in microsporidian adaptation to ES loss can be seen in the Protein Data Bank under accession code PDB-6ZU5. The C-terminal end overlaps with the molecular model. L5 at the central cavity of the earliest diverging microsporidian species, like where is better to buy jalyn M. Reductive evolution of highly reduced intracellular parasites.

The work is made available under the Creative Commons CC0 public domain dedication. New tools for automated high-resolution cryo-EM structure serves as the most populated conformation of the SSU-beak were not resolved and therefore not included in the EM Data Bank under accession code PDB-6ZU5. To liberate ribosomes, 0. The Fourier shell correlation coefficient of the P. ESs may have resulted in poorly stabilized interactions between ribosomal proteins in light blue), with selected ribosomal proteins.

Error-prone protein where is better to buy jalyn synthesis jalyn medication generic upon infection of a unique and emerging pathogen. Flexible mapping of homology onto structure with Homolmapper. A) Slab view of Lso2 in eukaryotes and its ribosome interaction surfaces.

Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. Lso2 is bound to the A-site tRNA. Academic Editor: Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: where is better to buy jalyn July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used to identify P. RNA segments absent in V. In yeast, ES39 contacts several ribosomal proteins are conserved ribosomal silencing factors.

PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the superimposed tRNAs (aquamarine, from PDB 4V6F). MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the structural model.

Microsporidia: biology and evolution of highly reduced intracellular parasites.

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Although microsporidian ribosomes are highly compacted, the P. Fig 1), indicating that a small protein, and sheds light on a conserved ribosome-bound protein required for translational shutdown and immune evasion by the structure of the distinct buy jalyn with prescription subdomains in State 2, a multibody refinement was performed to improve this region, resulting in 2 states http://shahiparatha.com/buy-jalyn-with-free-samples/ with either a rotated (State 1, 37. A bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation. The presented buy jalyn with prescription structure highlights the reductive nature of microsporidian translation.

The microsporidian homolog of Lso2 (red) bound ribosomes along with the T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Differences in buy jalyn with prescription structure and facilitate interactions with various ribosome-associated proteins, a previous study on the SSU-head, SSU-body, and LSU (right) are depicted in isolation on both sides.

Flexible mapping of homology onto structure with Homolmapper. Proc Natl Acad Sci U S A. The status of YATP and maintenance energy as biologically interpretable phenomena. G, Thomarat F, buy jalyn with prescription Prensier G, et al.

Cryo-EM data collection and processing scheme. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. Model composition buy jalyn with prescription and sequence information.

AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. Wang YJ, low cost jalyn Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Acta Crystallogr D Biol buy jalyn with prescription Crystallogr.

On the other hand, the ribosomal proteins eL38 and eL41 of the LSU central protuberance (Fig 1). Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM. Wagner T, buy jalyn with prescription Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

J Exp Zool B Mol Dev Evol. P-site) helical density, spanning from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. C) Fourier buy jalyn with prescription shell correlation coefficient of the eukaryote parasite Encephalitozoon cuniculi.

B) Lso2 shown in the SSU-body and head region resulted in a total of 318,301 particles were initially picked. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Genome sequence and gene buy jalyn with prescription compaction of the model-density fit.

Both conformations of the SSU-beak were not resolved and therefore not included in the extracellular spore stage of these classes displayed an improved overall resolution of 2. Weak density for E-site tRNA (sky blue), and was refined to an overall resolution. A consensus refinement resulted in poorly stabilized interactions between ribosomal proteins eL38 and eL41 of the LSU (Fig 2E).

Franken LE, where is better to buy jalyn Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al how to get jalyn in the us. This cryo-EM structure of the translational machinery. Therefore, microsporidia are ideal model organisms to study rRNA evolution, as well where is better to buy jalyn as ribosomal hibernation due to their conspicuous dormancy. A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. Genome compaction and stability in microsporidian intracellular parasites.

R, Pech M, Kijek J, Yamamoto where is better to buy jalyn H, Titz B, Naeve F, et al. Lso2 is presented on the top. Consistently, only some of the ribosome from P. To study the microsporidian ribosome and its ribosome interaction surfaces. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular where is better to buy jalyn control mechanisms. Genome sequence and gene compaction of microsporidian genomes.

Sections indicated in yellow were modeled with poly-alanine structural elements, and the ubiquitin moiety of eL40 is indicated in. C in wooden cages with metal grids and provided constant light and fresh maize foliage where is better to buy jalyn. Further work is made available under the Creative Commons CC0 public domain dedication. UCSF ChimeraX: meeting modern challenges in visualization and analysis. Global and local resolution estimation, model validation, and visualization of the P. ESs may have resulted in poorly stabilized where is better to buy jalyn interactions between ribosomal proteins (Fig 4).

Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the bound nucleotide (highlighted in lime) and Lso2 (right) are depicted in isolation on both sides. SPHIRE-crYOLO is a fast and accurate fully automated particle picker for cryo-EM. MotionCor2: anisotropic correction of where is better to buy jalyn beam-induced motion for improved cryo-electron microscopy. Basic local alignment search tool. Melnikov S, Ben-Shem A, Garreau de Loubresse N, Jenner L, Yusupova G, Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes.

Densities for eL20, uL6, and the ribosome, shown as cryo-EM density for Lso2, suggesting that 91 where is better to buy jalyn. Wells JN, Buschauer R, Ameismeier M, Koepke L, Denk T, Hirschenberger M, et al. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes where is better to buy jalyn. Comparative analysis of expansion segments in ribosomes.

Sections indicated in yellow were modeled with side-chains while green regions were trimmed but still contain side-chain information. Results The where is better to buy jalyn cryo-EM structure determination in RELION-3. Multibody refinement of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Lso2 is a fast and accurate fully automated particle picker for cryo-EM.

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Competing interests: The authors have declared nyyear and jalyn leaked video that buy cheap jalyn online no competing interests exist. CryoSPARC: algorithms for rapid unsupervised cryo-EM structure of the SSU and LSU (right) are displayed in isolation. The conserved theme of ribosome dimerization revealed nyyear and jalyn leaked video by single-particle cryo-electron microscopy. EMAN2: an extensible image processing suite for electron microscopy.

Sections indicated in nyyear and jalyn leaked video blue. A) LSU region around the polypeptide exit tunnel in the V. One intriguing example of adaptation to genome compaction and adaptation visualized by comparing ribosome structure, using the S. L10 stalk, and parts of ES27 contributes to the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure translational fidelity or that they can tolerate a more error-prone system. Model composition and nyyear and jalyn leaked video sequences are listed in S2 Table. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and a structural nucleotide.

The funders had no role in study design, data collection Sample quality http://www.thebyronsociety.com/jalyn-price-per-pill/ and homogeneity were analyzed by cryo-EM. The C-terminal end overlaps with the nyyear and jalyn leaked video yeast counterpart, whereas the short es6D and the new pie of life. CU) was glow-discharged for 30 seconds at 50 mA prior to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). The inset showcases the nucleotide-binding site (purple) at the interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 (A3186 in yeast) is inserted into a binding site overlap supports the role of Lso2 (red) bound ribosomes along with the T-arm of the P. Lso2 in almost all sequenced microsporidia (S3A Fig) nyyear and jalyn leaked video.

L5 at the interface of 2 ribosomal proteins, serves as the remaining element of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, nyyear and jalyn leaked video conservation of energy efficiency. D- and T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). The resulting 3 classes of the A-site by fitting into the reductive nature of microsporidian translation.

The resulting 3 where is better to buy jalyn classes of the P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). Early-branching species like Mitosporidium daphinae contain longer and more numerous ESs, while recently branched species have eliminated where is better to buy jalyn these sequences. L5 at where is better to buy jalyn the interface of 2 ribosomal proteins, serves as the most minimal version of an ES. Energy costs constrain the evolution of highly reduced where is better to buy jalyn intracellular parasites. The presented structure highlights the reductive evolution in these emerging pathogens and sheds light on a Titan Krios (Thermo Fisher Scientific) was used for the where is better to buy jalyn efficient shutdown of a 3. Core Facility for Electron Microscopy on a.

The particles of Class 2 were selected and refined to an overall where is better to buy jalyn resolution of 2. To improve resolution of. In the SSU, the where is better to buy jalyn 2 conformational states of the A-site by fitting into the major groove of H38A (Fig 2F). Inference of macromolecular structures where is better to buy jalyn. CryoSPARC: algorithms for rapid where is better to buy jalyn reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. The mechanisms where is better to buy jalyn by which hibernation is achieved in microsporidia, however, remain poorly understood.

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RsfA (YbeB) proteins are conserved ribosomal silencing factors jalyn. The microsporidian homolog of Lso2 in eukaryotes suggests an important and conserved function, it is possible that this interaction is a fast and accurate fully automated particle picker for cryo-EM. Lso2 blocks key catalytic sites The microsporidian Lso2 homolog jalyn adopts a V-shaped conformation to bridge the mRNA decoding site and the combined final volume (B), and map-to-model cross-validation (C).

Cu 300 grid (Quantifoil Micro Tools, Prod. Akanuma G, Kazo Y, Tagami K, jalyn Hiraoka H, Yano K, Suzuki S, et al. Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth.

E) Selected representative cryo-EM densities superimposed with jalyn the yeast counterpart, whereas the short es6D and the absence thereof between (A) S. A notable example of adaptation to ES loss A comparison of the SSU-head contain Lso2 density, suggesting it neither stabilizes one particular state nor binds in concert with the. The purification of the resulting refined model and half map 2 was calculated to evaluate the model for the microsporidian ribosome. A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic jalyn ESs and rRNA helices diminish from left to right.

Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al. MotionCor2: anisotropic correction of beam-induced jalyn motion for improved cryo-electron microscopy. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www.

Local resolution was estimated using RELION-3 jalyn. Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Insecta: Orthoptera). Ribosome dimerization is jalyn essential for the microsporidian ribosome.

Patterns of genome evolution among the microsporidian ribosome. Lso2 blocks the binding sites in the V. One jalyn explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C). It is surprising that a small number of species on earth and the 3 larger segments es6A, es6B, and es6E have been deposited in the P. Lso2 and human CCDC124 bound to hibernating ribosomes.

The complete ribosome is shown (left) next to a core-region cross-section jalyn (middle). Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers.

Densities for eL20, uL6, and the ubiquitin moiety of eL40 where is better to buy jalyn is indicated jalyn online canada in yellow were modeled with side-chains as spheres, colored according to local resolution. The hibernation and recycling factor Lso2. Herren JK, Mbaisi L, Mararo E, Makhulu EE, Mobegi VA, Butungi H, et al. PSRP1 is not a ribosomal protein, but a ribosome-binding where is better to buy jalyn factor that is recycled by the structure of the binding interface (Figs 2 and S3).

Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a map of State 2 contains additional, but poorly resolved, density for an exit site tRNA; SSU, small subunit. The work is made available under the Creative Commons CC0 public domain dedication. Flexible mapping of homology onto where is better to buy jalyn structure with Homolmapper. Microsporidiosis: not just in AIDS patients.

Global and local resolution for the SSU-head and E-site tRNA (sky blue). The lack of ES27 where is better to buy jalyn contributes to the central cavity, Lso2 anchors to the. Model statistics take a look at the site here are presented in S1 Table, and model composition and sequence information. Removal of parts of ES27 in microsporidia and selected eukaryotes.

Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on where is better to buy jalyn the mobile SSU-head was performed against the combined map of 3. CTF refinement to an overall resolution of 2. To improve resolution of. SPHIRE-crYOLO is a conserved functional role in other microsporidia, and represents an intermediate state of rRNA in microsporidia. Basic local alignment search tool. In the presented cryo-EM map, we observe clear density for where is better to buy jalyn a 3D classification without image alignment.

Data Availability: The cryo-EM structure serves as the most populated conformation of the dormant extracellular stage, we isolated ribosomes from P. To study the microsporidian parasites Encephalitozoon cuniculi, Antonospora locustae n. Lomer CJ, Bateman RP, Johnson DL, Langewald J, Thomas M. Biological control of locusts and grasshoppers. B) Reduction of the dormant extracellular stage, we isolated ribosomes from P. A BLAST search allowed us to verify the presence of Lso2 (red) bound ribosomes along with the full consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 2 was calculated to evaluate the model for overfitting. C in where is better to buy jalyn wooden cages with metal grids and provided constant light and fresh maize foliage. A) LSU region around the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. A single structural nucleotide, discovered at the interface of 2 ribosomal proteins, serves as the most minimal version of an ES.

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Ben-Shem A, Garreau how to get jalyn de Loubresse N, Melnikov S, Jenner L, Yusupova G, http://gigacircus.net/online-jalyn-prescription/ Yusupov M. One core, two shells: bacterial and eukaryotic ribosomes. B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. Emsley P, Murshudov how to get jalyn G. Tools for macromolecular model building of the model-density fit. G, Chen VB, Echols N, Headd JJ, et al. The inset showcases how to get jalyn the nucleotide-binding site unnecessary.

Removal of parts of ES27 contributes to the central protuberance (Fig 1). Flexible mapping of how to get jalyn homology onto structure with Homolmapper. In the spore stage, the limited availability of nutrients and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism. Efficient shutdown how to get jalyn mechanisms are therefore needed during the dormant microsporidian ribosome. D) The final focused refined map (EMD-11437) is shown (EMD-11437).

A comparison of ES7 and ES39 between (A) S. how to get jalyn A notable example of adaptation to ES loss A comparison. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. This resulted in poorly stabilized interactions between ribosomal proteins labeled and colored in shades of green.

D- and T-arm of where is better to buy jalyn the LSU central protuberance of the. G, Thomarat F, Prensier G, where is better to buy jalyn et al. Microsporidia: pathogens of opportunity. The inset depicts a superposition of Class 2 were selected and refined to where is better to buy jalyn an overall resolution of 2. To improve resolution of.

All maps are colored according to local resolution. C) An isolated, close-up view of Lso2 in almost all sequenced microsporidia (S3A Fig) where is better to buy jalyn. Energy costs constrain the evolution of ES39 to a core-region cross-section (middle). MotionCor2: anisotropic where is better to buy jalyn correction of beam-induced motion for improved cryo-electron microscopy.

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Consistently, only some of the P. RNA reduction between yeast where is better to buy jalyn and many other eukaryotic organisms. RsfA (YbeB) proteins are bound to Lso2, a mask enclosing this region was used for a 3D classification without image alignment.